TWISTING AND SWIVELING DOMAIN MOTIONS IN CAS9 TO RECOGNIZE TARGET DNA DUPLEXES, MAKE DOUBLE-STRAND BREAKS, AND RELEASE CLEAVED DUPLEXES

The CRISPR-associated protein 9 (Cas9) has been engineered as a precise gene editing tool to make double-strand breaks.CRISPR-associated protein 9 binds the folded guide RNA (gRNA) that serves as a binding scaffold to guide it to the target DNA duplex via a RecA-like strand-displacement mechanism but without ATP binding or hydrolysis.The target sea

Abstract As the importance of cell heterogeneity has begun to be emphasized, single-cell sequencing approaches are rapidly adopted to study cell heterogeneity and cellular evolutionary relationships of various cells, including stem cell populations.The hematopoietic stem and progenitor cell salamander chameleon (HSPC) compartment contains HSC hemat